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Topic: Crystal proteins as anti-nematode (anthelmintic) compounds

Hu, Y., Platzer, E.G., Bellier, A., and Aroian, R.V. (2010) Discovery of a highly synergistic anthelmintic combination that shows mutual hypersusceptibility. Proc Natl Acad Sci 107(13): 5955-5960. link

Hu* Y, Georghiou* SB, Kelleher AJ, Aroian RV, 2010 Bacillus thuringiensis Cry5B Protein Is Highly Efficacious as a Single-Dose Therapy against an Intestinal Roundworm Infection in Mice. PLoS Negl Trop Dis 4(3): e614. doi:10.1371/journal.pntd.0000614. link

Hu, Y., Xiao, S.H., and Aroian, R.V. (2009) The new anthelmintic tribendimidine is an L-type nicotinic acetylcholine receptor agonist, PLoS Negl Trop Dis 3(8): e499. doi:10.1371/journal.pntd.0000499. link

Li, X.Q., Tan, A., Voegtline, M., Bekele, S., Chen, C.S., and Aroian, R.V. (2008) Expression of Cry5B Protein from Bacillus thuringiensis in Plant Roots Confers Resistance to Root-Knot Nematode, Biological Control, 47(1) 97-102. pdf

Li, X.Q., Wei, J.Z., Tan, A., and Aroian, R.V., (2007) Resistance to root-knot nematode in tomato roots expressing a nematicidal Bacillus thuringiensis crystal protein, Plant Biotechnology Journal 5: pp. 455–464. pdf

Cappello, M., Bungiro, R.D., Harrison, L.M., Bischof, L.J., Griffitts, J.S., Barrows, B.D., and Aroian, R.V. (2006) A purified Bacillus thuringiensis crystal protein with therapeutic activity against the hookworm parasite Ancylostoma ceylanicum. Proc. Natl. Acad. Sci. 103(41): 15154-15159. pdf

Wei* J.Z., Hale* K., Carta L., Platzer E., Wong C., Fang S.C., and Aroian R.V. (2003) Bacillus thuringiensis Crystal proteins that target nematodes, Proc. Natl. Acad. Sci. 100: 2760-2765. pdf


Topic: Cellular defenses against and intoxication by pore-forming toxins

Chen C-S, Bellier A, Kao C-Y, Yang Y-L, Chen H-D, et al. 2010 WWP-1 Is a Novel Modulator of the DAF-2 Insulin-Like Signaling Network Involved in Pore-Forming Toxin Cellular Defenses in Caenorhabditis elegans. PLoS ONE 5(3): e9494. doi:10.1371/journal.pone.0009494. link

Bellier A., Chen C-S, Kao C-Y, Cinar HN, and Aroian RV (2009) Hypoxia and the Hypoxic Response Pathway Protect against Pore-Forming Toxins in C. elegans. PLoS Pathog 5(12): e1000689. doi: 10.1371/journal.ppat.1000689. pdf

Bischof L.J., Kao C.Y., Los F.C.O., Gonzalez M.R., Shen Z., Briggs S.P., van der Goot F.G., and Aroian R.V. (2008) Activation of the unfolded protein response is required for defenses against bacterial pore-forming toxin in vivo, PLoS Pathogens 4(10): e1000176. doi:10.1371/journal.ppat.1000176 . pdf

Bischof, L.J., Huffman, D.L., and Aroian, R.V. (2006) Assays for toxicity studies in C. elegans with Bt crystal proteins. Methods in Molecular Biology 351: 139-154.

Huffman D.L., Abrami* L., Sasik* R., Corbeil J., van der Goot F.G., and Aroian R.V. (2004) Mitogen-activated protein kinase pathways defend against bacterial pore-forming toxins. Proc. Natl. Acad. Sci. 101(30): 10995-11000. pdf


Topic: Development of Resistance to Crystal proteins

See also: Chen et al., 2010 and Bellier et al., 2009 (above)

Barrows, B.D., Haslam, S.M., Bischof, L.J., Morris, H.R., Dell, A., and Aroian, R.V. (2007) Resistance to Bt toxin in Caenorhabditis elegans from loss of fucose. J. Biol. Chem. 282: 3302-3311 . pdf

Barrows, B.D., Griffitts, J.S., and Aroian, R.V. (2006)Caenorhabditis elegans Carbohydrates in Bacterial Toxin Resistance. Methods in Enzymology 417: 340-358.

Griffitts J.S., Haslam, S.M., Yang, T., Garczynski, S.F., Mulloy, B., Morris, H., Cremer, P.S., Dell, A., Adang, M.J., and Aroian, R.V. (2005) Glycolipids as receptors for Bacillus thuringiensis crystal toxin. Science 307: 922-925. pdf

Griffitts J.S., Huffman D.L., Whitacre J.L., Barrows B.D., Marroquin L.D., Müller R., Brown J.R., Hennet T., Esko J.D., and Aroian R.V. (2003) Resistance to a bacterial toxin is mediated by removal of a conserved glycosylation pathway required for toxin - host interactions, J. Biol. Chem., 278(46): 45594-45602. pdf

Griffitts J.S., Whitacre J.L., Stevens D.E., and Aroian R.V. (2001) Bt toxin resistance from loss of a putative carbohydrate-modifying enzyme, Science, 293:860-864. pdf

Marroquin* L.D., Elyassnia* D., Griffitts J.S., Feitelson J.S. and Aroian R.V. (2000) Bacillus thuringiensis (Bt) toxin susceptibility and isolation of resistance mutants in the nematode Caenorhabditis elegans, Genetics, 155: 1693-1699. pdf


Topic: Reviews

Kao, C. Y., Los, F. C. O., and Aroian R.V. (2008) Nervous about immunity: neuronal signals control innate immune system. Nat Immunol. 9:1329-1330.

Aroian R. V. and van der Goot, F.G., (2007) Pore-forming toxins and cellular non-immune defenses (CNIDs).
Curr. Opin. Microbiol. 10:57-61. pdf

Barrows, B.D., Griffitts, J.S., and Aroian, R.V. (2007) Resistance is non-futile: Resistance to Crystal Toxin in the nematode Caenorhabditis elegans, J. Invert. Path.,95(3):198-200.

Griffitts, J.S. and Aroian, R.V. (2005) Many roads to resistance: How invertebrates adapt to Bt toxins. Bioessays 27: 614-624. pdf

Huffman D.L., Bischof L.J., Griffitts J.S., and Aroian R.V. (2004) Pore worms: Using Caenorhabditis elegans to study how bacterial toxins interact with their target hosts. Int. J. Med. Microbiol., 293: 599-607.


Topic: Embryonic Development (project retired in 2003)

Rappleye* C.A., Tagawa* A., Le Bot N., Ahringer J., Aroian R.V. (2003) Involvement of fatty acid pathways and cortical interaction of the pronuclear complex in Caenorhabditis elegans embryonic polarity, BMC Developmental Biology 3: 8.

Rappleye C.A., Tagawa A., Lyczak, R. Bowerman B., and Aroian R.V. (2002) The anaphase-promoting complex and separin are required for embryonic anterior-posterior axis formation, Developmental Cell 2(2): 195-206.

Tagawa A., Rappleye C., and Aroian R.V. (2001) pod-2, along with pod-1, defines a new class of genes required for polarity in the early Caenorhabditis elegans embryo, Developmental Biology, 233: 412-424.

Rappleye C., Paredez A., Smith C., McDonald K. and Aroian R.V. (1999) The coronin-like protein POD-1 is required for anterior-posterior axis formation and cellular architecture in the nematode Caenorhabditis elegans, Genes & Development, 13: 2838-2851.

*denotes equally contributing authors.

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